SCF protein (Mouse) (30R-2979)
Purified recombinant Mouse SCF protein
Overview
Overview
| Synonyms | KL protein, Steel factor protein, kit-ligand protein, Stem Cell factor protein |
|---|---|
| Specificity | Mouse |
| Species | Mouse |
| Protein Type | Recombinant |
| Applications | Functional Assay |
| Assay Information | A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate. The Functional Grade purified R4-6A2 antibody has been tested for neutralization of mouse IFN-? bioactivity |
Specifications
| Expression System | E.coli |
|---|---|
| Grade & Purity | > 98% pure |
| Method of Purification | SCF protein was purified by affinity chromatography. |
| Molecular Weight | 18 kDa |
| Form & Buffer | Supplied as a sterile liquid with 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, and 1.0% BSA. 0.22 um filtered. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Bioactivity | The ED50 of this protein, as measured by MC/9cell proliferation assay, is less than or equal to 65 ng/ml.This corresponds to a specific activity of greater than or equal to 1.5 x 10e4 Units/mg. |
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Storage & Safety
| Storage | Ships on dry ice.Upon receipt store at -70 deg C |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Mouse Stem Cell Factor is a hematopoietic growth factor and ligand for the KIT tyrosine kinase receptor, which exerts its activity in the early stages of hematopoiesis. Mouse SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures. |
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