SCF protein (Mouse) (30R-AS003x1)

Purified recombinant Mouse SCF protein

Synonyms MGF protein, Steel Factor protein, Kit ligand Precursor protein, KL-1 protein, C-kit ligand protein, Hematopoietic growth factor KL protein, SCF protein, DKFZp686F2250 protein, c-Kit Ligand protein, Mast cell growth factor protein, MGF protein, SF protein, Steel factor protein, Mast Cell Growth Factor protein, Kitl protein, Stem Cell Factor protein
Species Mouse
Protein Type Recombinant
Applications User optimized
Assay Information Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard.

Specifications

Residues Stem Cell Factor Mouse Recombinant protein containing 165 amino acids
Expression System E.coli
Grade & Purity > 98% pure
Method of Purification SCF protein (Mouse) was purified by a proprietary chromatographic technique.
Molecular Weight 18,309 Da
Form & Buffer Lyophilized from a concentrated (1 mg/ml) solution in Water containg 10 mM Na3PO4, pH6.5. Reconstitute in sterile water to a concentration not less that 100 ug/ml.

Usage & Assay Information

Bioactivity The ED50 as determined by the dose-dependant stimulation of Human TF-1 cell line is

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).

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Availability: In stock

Price: €1,594.74
Size: 500 ug
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