SCMH1 antibody (70R-13047)

Affinity purified Rabbit polyclonal SCMH1 antibody

Synonyms Polyclonal SCMH1 antibody, Anti-SCMH1 antibody, Scml3 antibody, SCMH-1, sex comb on midleg homolog 1 (Drosophila) antibody, SCMH 1 antibody, SCMH1, SCMH 1, SCMH-1 antibody, polycomb protein SCMH1 antibody, SCMH1 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen SCMH1 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 198 of SCMH1

Images

Immunofluorescent staining using SCMH1 antibody (70R-13047)

Immunofluorescence analysis of paraformaldehyde-fixed A549, using SCMH1 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification SCMH1 antibody was purified by antigen-affinity chromatography
Molecular Weight 65 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, IHC-P: 1:100-1:250, ICC:1:100-1:200, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using SCMH1 antibody (70R-13047)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed A549, using SCMH1 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using SCMH1 antibody (70R-13047)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A:Raji) using a 7.5 % SDS PAGE gel and SCMH1 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using SCMH1 antibody (70R-13047)</font></div>     | Immunohistochemical staining of paraffin-embedded Cal27 xenograft using SCMH1 antibody at a dilution of 1:100

Availability: In stock

Price: €323.11
Size: 100 ul
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