SEPHS2 antibody (70R-13124)

Affinity purified Rabbit polyclonal SEPHS2 antibody

Synonyms Polyclonal SEPHS2 antibody, Anti-SEPHS2 antibody, SEPHS-2, SEPHS-2 antibody, selenium donor protein 2 antibody, SEPHS2, SEPHS 2, selenide water dikinase 2 antibody, SPS2 antibody, selenophosphate synthetase 2 antibody, selenophosphate synthase 2 antibody, SEPHS2 antibody, SEPHS 2 antibody
Cross Reactivity Human
Applications ICC, IF, WB
Immunogen SEPHS2 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 186 and 412 of SEPHS2

Images

Immunofluorescent staining using SEPHS2 antibody (70R-13124)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SEPHS2 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification SEPHS2 antibody was purified by antigen-affinity chromatography
Molecular Weight 47 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop. Alternatively spliced transcripts have been identified, but their biological validity has not been determined.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using SEPHS2 antibody (70R-13124)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SEPHS2 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using SEPHS2 antibody (70R-13124)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A:HeLa S3) using a 7.5 % SDS PAGE gel and SEPHS2 antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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