SF20/IL25 protein (His tag) (80R-1101)
Purified recombinant Human SF20/IL25 protein
|Synonyms||IL27w protein, R337291 protein, IL27 protein, Interleukin25 protein, IL 27 protein, IL25 protein, Stromal cell derived growth factor SF20 protein, SF/IL25-20 protein, Interleukin 25 protein, Uncharacterized protein C19orf10 precursor., Stromal cell derived growth factor protein, EUROIMAGE1875335 protein, Stromal cell-derived growth factor Chromosome 19 open reading frame 10 protein, SF/IL25 20 protein, SF/IL25 20, R33729 1 protein, SF/IL25-20, SF20/IL25, IL 25 protein, UPF0556 protein, Interleukin 27 working designation protein, SF20 protein, C19orf10 protein|
Coomassie Blue stained SDS-PAGE of SF20/IL25 protein (His tag) (80R-1101)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||33-173 amino acids: MGSSHHHHHH SSGLVPRGSH MSEPTTVAFD VRPGGVVHSF SHNVGPGDKY TCMFTYASQG GTNEQWQMSL GTSEDHQHFT CTIWRPQGKS YLYFTQFKAE VRGAEIEYAM AYSKAAFERE SDVPLKTEEF EVTKTAVAHR PGAFKAELSK LVIVAKASRT EL|
|Grade & Purity||> 95% pure|
|Molecular Weight||18 kDa (162 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||SF20/25, originally identified as a product of bone marrow-derived stromal cells, was previously thought to support proliferation of lymphoid cells and was designated as interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. Recombinant human SF20/IL25 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.|
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