SF3B14 protein (His tag) (80R-1678)
Purified recombinant Human SF3B14 protein
|Synonyms||14 kDa subunit protein, Ht006 protein, HSPC175 protein, SFB14 3, P14 protein, SFB14 3 protein, SAP14 protein, Splicing factor 3B protein, SF3B14, CGI-110 protein, SFB14-3, SF3B14a, SFB14-3 protein|
Coomassie Blue stained SDS-PAGE of SF3B14 protein (His tag) (80R-1678)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-125 amino acids: MGSSHHHHHH SSGLVPRGSH MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK|
|Grade & Purity||> 95% pure|
|Molecular Weight||16.7 kDa (145aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 5mM DTT, 1mM EDTA and 30% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||SF3B14, also known as SAP14, is a 125 amino acid nuclear protein that is a component of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. Required for the splicing of pre-mRNA, SF3B14 enters the spliceosome and associates with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of U2 and U12 of the 17S U2 and the 18S U11/U12 snRNP complex. Recombinant human SF3B14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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