SGK1 protein (His tag) (80R-1988)
Recombinant human SGK1 protein (His tag)
Overview
Overview
| Synonyms | SGK1, Serine/threonine-protein kinase Sgk1 isoform 1 protein, SGK-1 protein, SGK 1 protein, SGK 1, SGK-1 |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of SGK1 protein (His tag) (80R-1988)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 60-431 amino acids: MGSSHHHHHH SSGLVPRGSH MISQPQEPEL MNANPSPPPS PSQQINLGPS SNPHAKPSDF HFLKVIGKGS FGKVLLARHK AEEVFYAVKV LQKKAILKKK EEKHIMSERN VLLKNVKHPF LVGLHFSFQT ADKLYFVLDY INGGELFYHL QRERCFLEPR ARFYAAEIAS ALGYLHSLNI VYRDLKPENI LLDSQGHIVL TDFGLCKENI EHNSTTSTFC GTPEYLAPEV LHKQPYDRTV DWWCLGAVLY EMLYGLPPFY SRNTAEMYDN ILNKPLQLKP NITNSARHLL EGLLQKDRTK RLGAKDDFME IKSHVFFSLI NWDDLINKKI TPPFNPNVSG PNDLRHFDPE FTEEPVPNSI GKSPDSVLVT ASVKEAAEAF LGFSYAPPTD SFL |
|---|---|
| Expression System | Hi-5 cells |
| Grade & Purity | > 90% pure |
| Molecular Weight | 44.5 kDa (393aa) |
| Tag/Conjugate | His tag |
| Form & Buffer | Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.2M NaCl,2mM DTT,0.1mM PMSF |
| Concentration | 0.25 mg/ml |
Usage & Assay Information
| Usage Recommendations | SGK1 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user. |
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Storage & Safety
| Storage | Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles. |
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General Information
| Biological Significance | SGK1, also known as serum-and glucocorticoid-regulated kinase, is a serine/threonine protein kinase and a member of the “AGC” subfamily, which includes protein kinases A, G, and C. This protein plays an important role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. It is activated in vitroby 3-phosphoinositide-dependent protein kinase-1 (PDK-1) and in vivoin response to signals that activate phosphatidylinositol (PI) 3-kinase. Recombinant human SGK1 protein was expressed with N-terminal His-tag in High-Five cells using baculovirus expression system and purified by using conventional chromatography techniques. |
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