SH3BP1 antibody (70R-13097)

Affinity purified Rabbit polyclonal SH3BP1 antibody

Synonyms Polyclonal SH3BP1 antibody, Anti-SH3BP1 antibody, SH3 domain-binding protein 1 antibody, 3BP-1 antibody, SHBP1 3 antibody, SH3BP1, ARHGAP43 antibody, SH3BP1 antibody, SHBP1-3 antibody, SH3-domain binding protein 1 antibody, SHBP1-3, SHBP1 3, SH3 domain binding protein 1 antibody
Cross Reactivity Human
Applications ICC, IF, WB
Immunogen SH3BP1 antibody was raised in rabbit using a synthetic peptide contain a sequence corresponding to a region within amino acids 639 and 651 of SH3BP1

Images

Immunofluorescent staining using SH3BP1 antibody (70R-13097)

Immunofluorescence analysis of methanol-fixed HeLa, using SH3BP1 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification SH3BP1 antibody was purified by antigen-affinity chromatography
Molecular Weight 76 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using SH3BP1 antibody (70R-13097)</font></div>     | Immunofluorescence analysis of methanol-fixed HeLa, using SH3BP1 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using SH3BP1 antibody (70R-13097)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Hela; B: JurKat) using a 7.5 % SDS PAGE gel and SH3BP1 antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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