Sheep anti Bovine IgG (H chain) (HRP) (43R-IS001hrp)
Sheep anti Bovine IgG (H chain) secondary antibody (HRP)
Overview
Overview
| Synonyms | Bovine Immuonglobulin G (H chain) antibody (HRP), Cow IgG (H chain) antibody (HRP) |
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| Specificity | Bovine |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, ICC, IHC, WB |
Specifications
| Host | Sheep |
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| Isotype | IgG |
| Method of Purification | Sheep anti Bovine IgG (H chain) (HRP) was purified by affinity chromatography. |
| Tag/Conjugate | HRP |
| Form & Buffer | PBS with 0.1% Pro-Clean 400. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, IHC: 1:200-1:500, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000 |
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Storage & Safety
| Storage | Store at 4 deg C. |
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General Information
| Biological Significance | There are five types of mammalian Ig heavy chain denoted by the Greek letters: alpha, delta, epsilon, gamma, and mu. The type of heavy chain present defines the class of antibody; these chains are found in IgA, IgD, IgE, IgG, and IgM antibodies, respectively. Distinct heavy chains differ in size and composition; alpha and gamma contain approximately 450 amino acids, while mu and epsilon have approximately 550 amino acids. |
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