Sheep anti Bovine IgM (HRP) (43C-CB0145)
Sheep anti Bovine IgM secondary antibody (HRP)
Overview
Overview
| Specificity | Bovine |
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| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, ICC, IHC, WB |
Specifications
| Host | Sheep |
|---|---|
| Isotype | IgG |
| Method of Purification | Sheep anti Bovine IgM (HRP) was purified by affinity chromatography. |
| Tag/Conjugate | HRP |
| Form & Buffer | Affinity purified and supplied in PBS with 0.2% BSA and 0.1% Pro-Clean 400. |
| Concentration | Batch dependent - please inquire should you have specific requirements |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, IHC: 1:200-1:500, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000 |
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Storage & Safety
| Storage | Store at 4 deg C. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | IgM is found in normal plasma at a concentration of 1.2 to 4.0 mg/ml. It is the first immunoglobulin produced during the immune response, the first antibody in neonates, and is involved in pathogenesis of autoimmune diseases. A diminished level of IgM is associated with Wiskott-Aldrich syndrome. |
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