Sheep anti Rabbit IgG (H + L) (Alk Phos) (43C-CB1170)

Sheep anti Rabbit IgG (H + L) secondary antibody (Alk Phos)

Synonyms Sheep anti Rabbit Immunoglobulin G (H + L) secondary antibody (Alk Phos)
Specificity Rabbit IgG (H + L)
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen Sheep anti-rabbit IgG (H+L) (Alk Phos) was raised in sheep using rabbit IgG whole molecule as the immunogen.

Images

Western blot analysis using Sheep anti Rabbit IgG (H + L) (Alk Phos) (43C-CB1170)

Western blot of Alkaline Phosphatase conjugated Sheep Anti-Rabbit IgG. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. None. Alkaline Phosphatase sheep secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..

Specifications

Host Sheep
Method of Purification Sheep anti Rabbit IgG (H + L) (Alk Phos) was purified by affinity chromatography.
Tag/Conjugate Alkaline Phosphatase
Form & Buffer 0.05M TRIS-HCl, pH 8.0, with 0.15M NaCl, 0.001M MgCl, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:15,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,000

Storage & Safety

Storage Store at 4 deg C. DO NOT FREEZE.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer.

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Images

  • Western blot analysis using Sheep anti Rabbit IgG (H + L) (Alk Phos) (43C-CB1170) | Western blot of Alkaline Phosphatase conjugated Sheep Anti-Rabbit IgG. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. None. Alkaline Phosphatase sheep secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..

Availability: In stock

Price: €258.73
Size: 1 mg
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