Sheep anti Rabbit IgG (H + L) (Alk Phos) (43C-CB1170)
Sheep anti Rabbit IgG (H + L) secondary antibody (Alk Phos)
Overview
Overview
| Synonyms | Sheep anti Rabbit Immunoglobulin G (H + L) secondary antibody (Alk Phos) |
|---|---|
| Specificity | Rabbit IgG (H + L) |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Sheep anti-rabbit IgG (H+L) (Alk Phos) was raised in sheep using rabbit IgG whole molecule as the immunogen. |
Images
Western blot analysis using Sheep anti Rabbit IgG (H + L) (Alk Phos) (43C-CB1170)
Western blot of Alkaline Phosphatase conjugated Sheep Anti-Rabbit IgG. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. None. Alkaline Phosphatase sheep secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..
Specifications
| Host | Sheep |
|---|---|
| Method of Purification | Sheep anti Rabbit IgG (H + L) (Alk Phos) was purified by affinity chromatography. |
| Tag/Conjugate | Alkaline Phosphatase |
| Form & Buffer | 0.05M TRIS-HCl, pH 8.0, with 0.15M NaCl, 0.001M MgCl, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:15,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,000 |
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Storage & Safety
| Storage | Store at 4 deg C. DO NOT FREEZE. |
|---|---|
| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. |
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