Skp protein (His tag) (80R-1352)

Purified recombinant E.coli Skp protein

Synonyms DNA binding 17 kDa protein protein, JW0173 protein, ompH protein, hlpA protein, Chaperone protein skp protein, Histone like protein HLP 1 protein, Seventeen kilodalton protein, Chaperone protein skp protein, ECK0177 protein, periplasmic chaperone protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of Skp protein (His tag) (80R-1352)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 21-161 amino acids: MGSSHHHHHH SSGLVPRGSH MADKIAIVNM GSLFQQVAQK TGVSNTLENE FKGRASELQR METDLQAKMK KLQSMKAGSD RTKLEKDVMA QRQTFAQKAQ AFEQDRARRS NEERGKLVTR IQTAVKSVAN SQDIDLVVDA NAVAYNSSDV KDITADVLKQ VK
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 17.9 kDa (162aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The Seventeen Kilodalton Protein (Skp) is a trimeric periplasmic chaperone that assists outer membrane proteins in their folding and insertion into membranes. This protein is required for the efficient release of ompA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of ompA into the outer membrane. Recombinant E. coli Skp, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of Skp protein (His tag) (80R-1352) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €206.99
Size: 100 ug
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