SMAD3 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMAD3 as the immunogen
Western blot analysis using SMAD3 antibody (70R-50027)
Western blot analysis of SMAD3 (pS204) expression in HeLa UV-treated (A); SP2/0 UV-treated (B); H9C2 UV-treated (C) whole cell lysates.
SMAD3 antibody
Western blot analysis using SMAD3 antibody (70R-50027)
Western blot analysis of SMAD3 (pS204) expression in HeLa UV-treated (A); SP2/0 UV-treated (B); H9C2 UV-treated (C) whole cell lysates.
Immunofluorescent analysis using SMAD3 antibody (70R-50027)
Immunofluorescent analysis of SMAD3 (pS204) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
SMAD3 antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
SMAD3 is a member of the SMAD family of proteins. It mediates the signals from the transforming growth factor beta (TGF-ß) superfamily ligands that regulate cell proliferation, differentiation and death.