SMAD3 antibody (70R-50027)

Purified Polyclonal SMAD3 antibody

Synonyms Polyclonal SMAD3 antibody, Anti-SMAD3 antibody, MADH3 antibody, Mothers against decapentaplegic homolog 3 antibody, MAD homolog 3 antibody, Mad3 antibody, Mothers against DPP homolog 3 antibody, hMAD-3 antibody, JV15-2 antibody, SMAD family member 3 antibody, SMAD 3 antibody, Smad3 antibody, hSMAD3 antibody
Specificity Recognizes endogenous levels of SMAD3 protein
Cross Reactivity Mouse, Rat, Chicken, Monkey, Pig, Sheep
Applications ICC, IF, IP, WB
Immunogen SMAD3 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMAD3 as the immunogen

Images

Western blot analysis using SMAD3 antibody (70R-50027)

Western blot analysis of SMAD3 (pS204) expression in HeLa UV-treated (A); SP2/0 UV-treated (B); H9C2 UV-treated (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification SMAD3 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance SMAD3 is a member of the SMAD family of proteins. It mediates the signals from the transforming growth factor beta (TGF-ß) superfamily ligands that regulate cell proliferation, differentiation and death.

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Images

  • 

Western blot analysis using SMAD3 antibody (70R-50027)



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Western blot analysis of SMAD3 (pS204) expression in HeLa UV-treated (A); SP2/0 UV-treated (B); H9C2 UV-treated (C) whole cell lysates.
  • 

Immunofluorescent analysis using SMAD3 antibody (70R-50027)



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Immunofluorescent analysis of SMAD3 (pS204) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €233.95
Size: 100 ul
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