SNAP25 protein (30R-1011)
Purified recombinant Human SNAP25 protein
|Synonyms||Synaptosomal-associated protein 25kDa protein, resistance to inhibitors of cholinesterase 4 homolog protein, bA416N4.2 protein, RIC 4, FLJ23079 protein, SNAP-25 protein, SNAP 25, RIC4 protein, SNAP25, SNAP-25 protein, SNAP-25, SNAP 25 protein, SNAP 25 protein, super protein protein, RIC-4 protein, synaptosomal-associated protein 25 protein, SNAP25 protein, synaptosomal-associated 25 kDa protein protein, SUP protein, SEC9 protein, dJ1068F16.2 protein|
Coomassie Blue stained SDS-PAGE of SNAP25 protein (30R-1011)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERC EGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQP RITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK|
|Grade & Purity||> 95% pure|
|Molecular Weight||23 kDa (207 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl, pH7.5, containing 50mM NaCl, 5mMDTT, 1mM EDTA, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||SNAP-25 is a presynaptic plasma membrane protein essential for synaptic vesicle fusion and neurotransmitter release . The membrane proteins SNAP-25, synaptobrevin and syntaxin make up the core complex (SNARE) essential for docking and holding synaptic vesicles at the pre synaptic membrane. SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques.|
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