SP100 protein (His tag) (80-1387)
Purified recombinant SP100 protein (His tag)
Overview
Overview
| Synonyms | Nuclear autoantigen Sp100, SP100 Nuclear Antigen, Nuclear dot-associated Sp100 protein, Speckled 100 kDa protein |
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| Species | Human |
| Protein Type | Recombinant |
Specifications
| Residues | Amino acids1-474 |
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| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Method of Purification | Purified using proprietary chromatographic techniques |
| Molecular Weight | 57 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in PBS buffer with 2mM DTT, 1mM PMSF, 1mM EDTA and 0.02% SDS |
Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Together with PML, SP100 tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. SP100 functions as a transcriptional coactivator of ETS1 and ETS2. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. SP100 may also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. |
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| Expression | SP100 is widely expressed in humans. Tissue and Cell Localization: Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines. |
| Implications in Disease | SP100 is one of the major tumor suppressors involved in cellular differentiation. Mutations in expression have been linkedto multiple carcinomas as well as Primary Biliary Cirrhosis (PBC), a chronic disease that affects the bile ducts within the liver |
| Sequence Information | SP100 is composed of 879 amino acids with a molecular weight of 100417 Da. It contains 2 HMG box DNA-binding domains, 1 HSR and 1 SAND domain. The HSR domain is important for the nuclear body targeting as well as for the dimerization. |
| Post-Translational Modifications | Sumoylation of SP100 depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting. Ubiquitination occurs at Lys151. |
| Database Links | Entrez Gene: 6672 Human, Omim: 604585 Human, SwissProt: P23497 Human, Unigene: 369056 Human |
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