SRP14 protein (His tag) (80R-1560)
Purified recombinant Human SRP14 protein
|Synonyms||Signal recognition particle 14 kDa protein protein, SRP 14 protein, SRP14, DKFZp468D2027, SRP-14 protein, SRP 14, SRP-14|
Coomassie Blue stained SDS-PAGE of SRP14 protein (His tag) (80R-1560)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-136 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMVLLES EQFLTELTRL FQKCRTSGSV YITLKKYDGR TKPIPKKGTV EGFEPADNKC LLRATDGKKK ISTVVSSKEV NKFQMAYSNL LRANMDGLKK RDKKNKTKKT KAAAAAAAAA PAAAATAATT AATTAATAAQ|
|Grade & Purity||> 85% pure|
|Molecular Weight||17.1 kDa (160aa) confirmed by MALDI-TOF (molecular size on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||SRP14 is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum. The “Alu domain” of SRP comprises the heterodimer of the SRP9 and SRP14 proteins, which are bound to the 5' and 3' terminal sequences of SRP RNA. SRP9/14 binding may be crucial to the transcription, maturation, nucleolus localization and transport of SRP RNA. Recombinant human SRP14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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