Stop/Elution Solution (85R-123)
Elution or stop reagent for use in immunoassays
Overview
Overview
| Synonyms | Stop Solution, Elution Reagent |
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Specifications
| Form & Buffer | Ready-to-use reagent for stopping developed enzymatic reaction with 3,3',5,5'tetramethylbenzidine (TMB) |
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Storage & Safety
| Storage | Store at 15-25 deg C. |
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General Information
| Biological Significance | Many immunoassays make use of enzymes as labels. Following antibody binding of target a suitable substrate is added to the reaction vessel. The signal arising from enzyme activity on this substrate can then be recorded. To stop enzyme activity a volume of stop solutiong specific for the enzyme/substrate pair is added to the reaction vessel. This will stop the reaction by altering the conditions within the vessel away from the optimal conditions of enzyme activity. |
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