Streptavidin antibody (FITC) (60C-CF0201)
Rabbit polyclonal Streptavidin antibody (FITC) conjugated
Overview
Overview
| Synonyms | Polyclonal Streptavidin antibody, Anti-Streptavidin antibody, Streptavidin antibody |
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| Specificity | Streptomyces avidinii streptavidin |
| Cross Reactivity | To be determined by end-user |
| Applications | FC, IF |
| Immunogen | Streptavidin antibody (FITC) was raised in rabbit using streptavidin as the immunogen. |
Images
Western Blot analysis using Streptavidin antibody (FITC) (60C-CF0201)
Streptavidin and Biotin conj ugated Rabbit anti-Trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of
Specifications
| Host | Rabbit |
|---|---|
| Method of Purification | Streptavidin antibody (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis. |
| Tag/Conjugate | FITC |
| Form & Buffer | IgG fraction supplied as a liquid in 20mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.2, with 10 mg/ml BSA (IgG and Protease free) as a stabilizer, and 0.01% NaN3 as a preservative. |
Usage & Assay Information
| Usage Recommendations | FC: 1:2,000-1:10,000, IF: 1:500-1:2,500, OTHER: FC: 1:2,000-1:10,000 |
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Storage & Safety
| Storage | Store at 4 deg C or aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin (also known as vitamin B7); the dissociation constant (kDa) of the biotin-streptavidin complex is on the order of ˜10-14 mol/L, making it one of the strongest non-covalent interactions known in nature. It is used extensively in molecular biology and bionanotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants, detergents and proteolytic enzymes. |
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