Streptavidin antibody (HRP) (60C-CF0204)

Rabbit polyclonal Streptavidin antibody (HRP) conjugated

Synonyms Polyclonal Streptavidin antibody, Anti-Streptavidin antibody, Streptavidin antibody
Specificity Streptomyces avidinii streptavidin
Cross Reactivity No cross reactivity withAvidin
Applications Dot Blot, ELISA, WB
Immunogen Streptavidin antibody (HRP) was raised in rabbit using streptavidin as the immunogen.

Specifications

Host Rabbit
Method of Purification Streptavidin antibody (HRP) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Tag/Conjugate HRP
Form & Buffer IgG fraction supplied as a liquid in 10mM Potassium Phosphate and 75mM NaCl, pH 7.2 with 5mg/ml BSA (IgG and Protease free) and 0.005% Gentamicin Sulfate
Concentration 10 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:20,000-1:100,000

Storage & Safety

Storage Store at 4 deg C short term. Aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.

General Information

Biological Significance Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin (also known as vitamin B7); the dissociation constant (kDa) of the biotin-streptavidin complex is on the order of ˜10-14 mol/L, making it one of the strongest non-covalent interactions known in nature. It is used extensively in molecular biology and bionanotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants, detergents and proteolytic enzymes.

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Availability: In stock

Price: €381.04
Size: 1 mg
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