Streptavidin antibody (HRP) (60C-CF0204)
Rabbit polyclonal Streptavidin antibody (HRP) conjugated
Overview
Overview
| Synonyms | Polyclonal Streptavidin antibody, Anti-Streptavidin antibody, Streptavidin antibody |
|---|---|
| Specificity | Streptomyces avidinii streptavidin |
| Cross Reactivity | No cross reactivity withAvidin |
| Applications | Dot Blot, ELISA, WB |
| Immunogen | Streptavidin antibody (HRP) was raised in rabbit using streptavidin as the immunogen. |
Images
Western Blot analysis using Streptavidin antibody (HRP) (60C-CF0204)
Streptavidin and Biotin conj ugated Rabbit anti-Trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of
Specifications
| Host | Rabbit |
|---|---|
| Method of Purification | Streptavidin antibody (HRP) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis. |
| Tag/Conjugate | HRP |
| Form & Buffer | IgG fraction supplied as a liquid in 10mM Potassium Phosphate and 75mM NaCl, pH 7.2 with 5mg/ml BSA (IgG and Protease free) and 0.005% Gentamicin Sulfate |
| Concentration | 10 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:20,000-1:100,000 |
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Storage & Safety
| Storage | Store at 4 deg C short term. Aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use. |
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General Information
| Biological Significance | Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin (also known as vitamin B7); the dissociation constant (kDa) of the biotin-streptavidin complex is on the order of ˜10-14 mol/L, making it one of the strongest non-covalent interactions known in nature. It is used extensively in molecular biology and bionanotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants, detergents and proteolytic enzymes. |
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