Streptavidin protein (Alk Phos) (65C-CE0302)
Purified homogeneous preparation of Alkaline Phosphatase conjugated Streptavidin protein
|Protein Type||Binding Protein|
|Applications||ELISA, IHC, WB|
Western Blot analysis using Streptavidin protein (Alk Phos) (65C-CE0302)
Lane 1: Guinea Pig IgG (biotin). Load: 50 ng per lane. Secondary antibody: Streptavidin (Alk Phos) at 1:1, 000 for 60 min at RT. Block for 30 min at RT.
|Method of Purification||Streptavidin protein (Alk Phos) was purified by Protein A affinity chromatography.|
|Form & Buffer||0.05M TRIS-HCl, pH 8.0, 0.15M NaCl, 0.001M MgCl, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:8,000-1:32,000, IHC: 1:200-1:1,000, WB: 1:1,000-1:4,000|
Storage & Safety
|Storage||Store at 4 deg C. DO NOT FREEZE.|
|Biohazard Information||This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.|
|Biological Significance||Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin (also known as vitamin B7); the dissociation constant (kDa) of the biotin-streptavidin complex is on the order of ˜10-14 mol/L, making it one of the strongest non-covalent interactions known in nature. It is used extensively in molecular biology and bionanotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants, detergents and proteolytic enzymes.|
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