Streptavidin protein (Alk Phos) (65C-CE0302)
Purified homogeneous preparation of Alkaline Phosphatase conjugated Streptavidin protein
Overview
Overview
| Species | Streptomyces avidinii |
|---|---|
| Protein Type | Binding Protein |
| Applications | ELISA, IHC, WB |
Images
Western Blot analysis using Streptavidin protein (Alk Phos) (65C-CE0302)
Lane 1: Guinea Pig IgG (biotin). Load: 50 ng per lane. Secondary antibody: Streptavidin (Alk Phos) at 1:1, 000 for 60 min at RT. Block for 30 min at RT.
Specifications
| Method of Purification | Streptavidin protein (Alk Phos) was purified by Protein A affinity chromatography. |
|---|---|
| Tag/Conjugate | Alkaline Phosphatase |
| Form & Buffer | 0.05M TRIS-HCl, pH 8.0, 0.15M NaCl, 0.001M MgCl, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:8,000-1:32,000, IHC: 1:200-1:1,000, WB: 1:1,000-1:4,000 |
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Storage & Safety
| Storage | Store at 4 deg C. DO NOT FREEZE. |
|---|---|
| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin (also known as vitamin B7); the dissociation constant (kDa) of the biotin-streptavidin complex is on the order of ˜10-14 mol/L, making it one of the strongest non-covalent interactions known in nature. It is used extensively in molecular biology and bionanotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants, detergents and proteolytic enzymes. |
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