Streptavidin protein (allophycocyanin) (80R-2359)
Purifed Streptavidin protein (allophycocyanin)
Overview
Overview
| Synonyms | SA protein, SA-APC protein, SA-APC protein, Sav protein |
|---|---|
| Species | Streptomyces avidinii |
| Protein Type | Binding Protein |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. |
Specifications
| Tag/Conjugate | Allophycocyanin |
|---|---|
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 200 ug/mL |
Usage & Assay Information
| Usage Recommendations | 20 ul (0.125 ug)/test |
|---|
Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity. |
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