Streptavidin protein (His tag) (80R-1063)
Purified recombinant Streptomyces avidinii Streptavidin protein
Overview
Overview
| Synonyms | Streptavidin protein, SA V2 protein, Streptomyces avidinii streptavidin protein, Streptavidin V2., Streptavidin V1 protein, SA V1 protein |
|---|---|
| Species | Streptomyces avidinii |
| Protein Type | Binding Protein |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of Streptavidin protein (His tag) (80R-1063)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 25-183 amino acids: MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ |
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| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 17.5 kDa (167 aa), confirmed by MALDI-TOF. |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris, pH 7.5. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E. coli and purified by using conventional chromatography techniques. |
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