Streptolysin O protein (30-1022)
Purified recombinant Streptolysin O protein
Overview
Overview
| Synonyms | SLO protein, High purity SLO |
|---|---|
| Species | Bacterial |
| Protein Type | Recombinant |
Images
Specifications
| Residues | Amino acids 34-5712of Accession numberQ8P2T7-1.Expressed with N terminal 6 X Histidine tag. |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 99% pure |
| Form & Buffer | Supplied in liquid form in 20 mM phosphate buffer (pH 8.5), 150mM NaCl, 1mM DTT, 1mM PMSFcontaining 50% glycerol. |
Usage & Assay Information
| Bioactivity | Tested for antigenic activity using Labcare Tubidometry kit and found to be >1250 IU per mg of protein. Its biological activity is in the range of native protein (~500000 HU/ mg). |
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Storage & Safety
| Storage | Aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | Use only in In-Vitro research or products. All bio-safety precautions must be practiced while handling. |
General Information
| Biological Significance | Streptolysin is a streptococcal hemolytic exotoxin. Types include Streptolysin O (SLO), which is oxygen-labile, Streptolysin O is hemolytically active only in a reversibly reduced state unlike Streptolysin S which is stable in the presence of oxygen. Another difference is that that SLO is antigenic while SLS is non antigenic due to its small size. |
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| Sequence Information | NKQNTASTET TTTNEQPKPE SSELTTEKAG QKTDDMLNSN DMIKLAPKEM PLESAEKEEK KSEDKKKSEE DHTEEINDKI YSLNYNELEV LAKNGETIEN FVPKEGVKKA DKFIVIERKK KNINTTPVDI SIIDSVTDRT YPAALQLANK GFTENKPDAV VTKRNPQKIH IDLPGMGDKA TVEVNDPTYA NVSTAIDNLV NQWHDNYSGG NTLPARTQYT ESMVYSKSQI EAALNVNSKI LDGTLGIDFK SISKGEKKVM IAAYKQIFYT VSANLPNNPA DVFDKSVTFK ELQRKGVSNE APPLFVSNVA YGRTVFVKLE TSSKSNDVEA AFSAALKGTD VKTNGKYSDI LENSSFTAVV LGGDAAEHNK VVTKDFDVIR NVIKDNATFS RKNPAYPISY TSVFLKNNKI AGVNNRTEYV ETTSTEYTSG KINLSHRGAY VAQYEILWDE INYDDKGKEV ITKRRWDNNW YSKTSPFSTV IPLGANSRNI RIMARECTGL AWEWWRKVID ERDVKLSKEI NVNISGSTLS PYGSITYK- |
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