Streptolysin O protein (Stabilized) (30-1023)
Purified recombinant stabilized Human Streptolysin O protein
Overview
Overview
| Synonyms | SLO protein, Stabilized SLO protein, Glutaraldehyde stabilized Streptolysin O protein |
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| Species | Bacterial |
| Protein Type | Recombinant |
| Assay Information | During stabilization procedure SLO molecule has cross-linked with Glutaraldehyde. Glutaraldehyde stabilization is required for coupling reaction with latex particle. |
Specifications
| Expression System | E.coli |
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| Grade & Purity | Highly pure |
| Method of Purification | Stabilized SLO protein was stabilized using glutaraldehyde and purified by metal affinity chromatography. |
| Form & Buffer | Supplied in liquid form in 20 mM TRIS-HCl, 150mM NaCl (pH 8.2 + 0.2) containing 0.09% Sodium Azide. |
Usage & Assay Information
| Bioactivity | Antigenic activity was determinedto be >1250 IU per mg of protein byLabcare Tubidometry kit. |
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Storage & Safety
| Storage | Aliquot and store at -70 deg C. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | Use only in In-Vitro research or products. All bio-safety precautions must be practiced while handling. |
General Information
| Biological Significance | Streptolysin is a streptococcal hemolytic exotoxin. Types include Streptolysin O (SLO), which is oxygen-labile, Streptolysin O is hemolytically active only in a reversibly reduced state unlike Streptolysin S which is stable in the presence of oxygen. Another difference is that that SLO is antigenic while SLS is non antigenic due to its small size. |
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