Syntaxin 1A protein (30R-1094)
Purified recombinant Human Syntaxin 1A protein
|Synonyms||STX1 protein, HPC 1 protein, Syntaxin A-1 protein, Syntaxin 1A protein, Neuron-specific protein HPC-1 protein, Syntaxin A-1, Syntaxin-1A protein, Neuron specific protein HPC1 protein, Syntaxin 1A brain., Syntaxin 1A, STX1A protein, Syntaxin A 1 protein, Syntaxin A 1, STX1 protein|
Coomassie Blue stained SDS-PAGE of Syntaxin 1A protein (30R-1094)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-226 amino acids: MKDRTQELRT AKDSDDDDDV AVTVDRDRFM DEFFEQVEEI RGFIDKIAEN VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQ|
|Grade & Purity||> 95% pure|
|Molecular Weight||26.1 kDa (226 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris, pH 7.5, containing 1mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Syntaxin is membrane integrated Q-SNARE protein participating in exocytosis. Syntaxin is composed of an N-terminal regulatory domain (Habc), a SNARE domain (known as H3), and a single C-terminal transmembrane domain. The SNARE (H3) domain binds to both synaptobrevin and SNAP-25 forming the core SNARE complex. Recombinant syntaxin 1A protein (1-226aa) contains N-terminal domain(Habc) and t-SNARE domain (H3 domain) and this protein was overexpressed in E. coli and purified by using the conventional column chromatography techniques.|
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