TAF2 antibody (10R-7203)
Mouse monoclonal TAF2 antibody
Overview
Overview
| Synonyms | Monoclonal TAF2 antibody, Anti-TAF2 antibody, CIF150 antibody, TAF2B antibody, TAFII150 antibody, TAF2, TAF 2, TAF-2, TAF 2 antibody, TAF-2 antibody |
|---|---|
| Cross Reactivity | Human |
| Applications | WB |
| Immunogen | TAF2 antibody was raised in Mouse using Human recombinant protein fragment corresponding to amino acids 782-1121 of human TAF2 produced in E.coli as the immunogen. |
Images
Western Blot analysis using TAF2 antibody (10R-7203)
Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TAF2 protein (Right) or empty vector (Left) detected with TAF2 antibody
Specifications
| Host | Mouse |
|---|---|
| Clone | 2A5 |
| Isotype | IgG1 |
| Method of Purification | TAF2 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| Concentration | 0.5-1 mg/ml |
Usage & Assay Information
| Usage Recommendations | WB: 1:2000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. |
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