TAP antibody (70R-12640)

Affinity purified Rabbit polyclonal TAP antibody

Synonyms Polyclonal TAP antibody, Anti-TAP antibody, MEX67 antibody, TAP antibody, DKFZp667O0311 antibody, NXF1 antibody, tip associating protein antibody, nuclear RNA export factor 1 antibody, tip-associating protein antibody, tip-associated protein antibody, mRNA export factor TAP antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, WB
Immunogen TAP antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 449 and 619 of TAP

Images

Western blot analysis using TAP antibody (70R-12640)

Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and TAP antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification TAP antibody was purified by Protein A chromatography
Molecular Weight 70 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 10.00 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using TAP antibody (70R-12640)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and TAP antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using TAP antibody (70R-12640)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAP antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using TAP antibody (70R-12640)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 7.5 % SDS PAGE gel and TAP antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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