TCR alpha antibody (70R-12400)

Rabbit polyclonal TCR alpha antibody

Synonyms Polyclonal TCR alpha antibody, anti-TCR alpha antibody, T cell receptor alpha antibody, T cell antigen receptor antibody, TCRA antibody, TCRD antibody
Cross Reactivity Human, Mouse, Rat
Applications IHC, IP, WB
Immunogen TCR alpha antibody was raised in Rabbit using synthetic peptide surrounding amino acid 138 of human TCR alpha as the immunogen.
Assay Information TCR alpha Blocking Peptide, catalog no. 33R-11053, is also available for use as a blocking control in assays to test for specificity of this TCR alpha antibody

Images

Western Blot analysis using TCR alpha antibody (70R-12400)

Western blot analysis of TCR alpha expression in Jurkat cell lysate.

Specifications

Host Rabbit
Isotype IgG
Method of Purification TCR alpha antibody was purified by affinity chromatography
Form & Buffer Supplied in PBS buffer containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal
Concentration 200 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.5-4 ug/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance TCR recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma and delta chains. The vast majority of circulating T cells express the alpha/beta heterodimer while roughly 2-5% express the gamma/delta heterodimer. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR.

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Images

  • <div><font face=Calibri size=2>Western Blot analysis using TCR alpha antibody (70R-12400)</font></div>     | Western blot analysis of TCR alpha expression in Jurkat cell lysate.

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