TGF beta 3 protein (30R-AT028)
Purified recombinant Human TGF beta 3 protein
Overview
Overview
| Synonyms | Transforming Growth Factor-beta3 protein, TGF beta-3 protein, TGFB3 protein, TGF beta-3, TGF-3b protein, TGF beta 3, TGF beta 3 protein, TGF beta-3 protein, TGF beta 3 |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | User optimized |
| Assay Information | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a calibrated solution of TGF-b 3 as a Reference Standard. |
Specifications
| Residues | TGF-b3 Human Recombinant protein containing 112 amino acids. |
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| Expression System | E.coli |
| Grade & Purity | > 99% pure |
| Method of Purification | TGF beta 3 protein was purified by a proprietary chromatographic technique. |
| Molecular Weight | 25, 445 Da |
| Form & Buffer | Supplied as a liquid with 20% Ethanol and 0.12% CH3COOH. Please Note: Buffer may change with new Lots. |
Usage & Assay Information
| Bioactivity | The ED50 as determined by the cell toxicity assay using the WHO Standard 98/608 as a direct comparison is |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that occur during embryonic development. Three TGF Betas have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. |
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