Thioredoxin 1 protein (His tag) (80R-1313)

Purified recombinant E.coli Thioredoxin 1 protein

Synonyms Thioredoxin 1, Thioredoxin -1, Thioredoxin 1 protein, Thioredoxin -1 protein, fipA protein, Thioredoxin 1, dasC protein, tsnC protein,
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 2ug of recombinant E.coli thioredoxin into reaction cocktail and mix immediately. 3. Incubate at 25 DEG C for 20 minutes. 4. Record the increase in A650nm for 15 minutes.

Specifications

Residues 2-109 amino acids: MHHHHHHMGS DKIIHLTDDS FDTDVLKADG AILVDFWAEW CGPCKMIAPI LDEIADEYQG KLTVAKLNID QNPGTAPKYG IRGIPTLLLF KNGEVAATKV GALSKGQLKE FLDANLAGS
Expression System E.coli
Grade & Purity > 85% pure
Molecular Weight 12.9 kDa (119aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 1mM DTT.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Specific activity is 16~18 A650/min/mg and was obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Thioredoxin 1(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant E. coli TXN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Availability: In stock

Price: €239.92
Size: 50 ug
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