Thioredoxin1 protein (30R-1066)

Purified recombinant Human Thioredoxin1 protein

Synonyms ATL derived factor protein, TXN protein protein, Thioredoxin 1 protein, TR-I protein, TRX1 protein, TXN protein, TRX protein, , DKFZp686B1993 protein, Thioredoxin1, Surface associated sulphydryl protein protein, Thioredoxin protein, Thioredoxin -1 protein, Thioredoxin I protein, TRX 1 protein, Thioredoxin-2 protein, Thioredoxin-1 protein, ADF protein, SASP protein, Thioredoxin 1, TRDX protein, TRX2 protein, MGC61975 protein, Thioredoxin -1, Thioredoxin-1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 1ml reaction cocktail in a suitable container. The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately. 3. Incubate at 25 DEG C for 20 minutes. 4. Record the increase in A650nm for 15 minutes

Images

Coomassie Blue stained SDS-PAGE of Thioredoxin1 protein (30R-1066)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 11.7 kDa (105 aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in PBS, pH 7.4.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques

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Images

  • Coomassie Blue stained SDS-PAGE of Thioredoxin1 protein (30R-1066) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €169.35
Size: 100 ug
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