TMB ELISA Substrate (42-TB08)
High sensitivity HRP microwell substrate (containing TMB)
Usage & Assay Information
| Usage Recommendations | Optimal conditions to be determined by end user. This product should be allowed to equilibrate to room temperature (25 |
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Storage & Safety
| Storage | Store at 4 deg C. |
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General Information
| Biological Significance | This HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution. Unreacted substrate should be colorless or very light yellow in appearance. When this substrate system is reacted with peroxidase, a soluble blue reaction product is obtained. The reaction can be stopped using appropriate stop solution, producing a soluble yellow or soluble blue reaction product, depending upon the stop reagent used, which is stable for at least 1 hour. |
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