TMB Substrate (85R-117)
High sensitivity TMB Substrate ready to use in immunoassays
Storage & Safety
| Storage | Store at 4 deg C. |
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General Information
| Biological Significance | TMB Substrate for ELISA is a peroxidase substrate containing 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide in an aqueous buffer formulation. This product is supplied as a one component ready-to-use reagent. Intact substrate is clear colorless to slightly yellowish in appearance. Reaction with peroxidase results in converting substrate into soluble blue (~ 650 nm) product that after stopping with strong acid further turns to super-oxidized soluble yellow product. Yellow product can be measured at 450 nm (measuring at 450 nm vs. 620-650 nm reference wavelength will additionally improve readout in ELISA). |
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