TMB Substrate (85R-118)
High sensitivity TMB Substratefor usein ELISA
Overview
Overview
| Synonyms | 3',3',5,5'-tetramethylbenzidine substrate, TMB |
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Specifications
| Form & Buffer | Peroxidase substrate containing 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide in an aqueous buffer formulation. This product is supplied as a one component ready-to-use reagent. |
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Storage & Safety
| Storage | Store at 4 deg C. |
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General Information
| Biological Significance | TMB Substrate for ELISA is a peroxidase substrate containing 3,3\',5,5\'-tetramethylbenzidine (TMB) and hydrogen peroxide in an aqueous buffer formulation. This product is supplied as a one component ready-to-use reagent. Intact/unreacted substrate is clear colorless to slightly yellowish in appearance. Reaction with peroxidase results in converting substrate into soluble blue (~ 650 nm) product which after stopping with strong acid further turns to super-oxidized soluble yellow product. Yellow product can be measured at 450 nm (measuring at 450 nm vs. 620-650 nm reference wavelength will additionally improve readout pattern in ELISA. |
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