TNF alpha antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TNF alpha as the immunogen
Western blot analysis using TNF alpha antibody (70R-50493)
Western blot analysis of TNF alpha expression in HeLa (A); NIH3T3 (B); mouse heart (C); rat brain (D) whole cell lysates.
TNF alpha antibody
Western blot analysis using TNF alpha antibody (70R-50493)
Western blot analysis of TNF alpha expression in HeLa (A); NIH3T3 (B); mouse heart (C); rat brain (D) whole cell lysates.
Immunohistochemical analysis using TNF alpha antibody (70R-50493)
Immunohistochemical analysis of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Specifications
Host
Rabbit
Method of Purification
TNF alpha antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide