TNF alpha antibody (70R-50493)

Purified Polyclonal TNF alpha antibody

Synonyms Polyclonal TNF alpha antibody, Anti-TNF alpha antibody, TNFA antibody, TNFSF2 antibody, Tumor necrosis factor antibody, Cachectin antibody, TNF-alpha antibody, Tumor necrosis factor ligand superfamily member 2 antibody, TNF-a antibody
Specificity Recognizes endogenous levels of TNF alpha protein
Cross Reactivity Mouse, Rat, Monkey, Pig
Applications IHC, WB
Immunogen TNF alpha antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TNF alpha as the immunogen

Images

Western blot analysis using TNF alpha antibody (70R-50493)

Western blot analysis of TNF alpha expression in HeLa (A); NIH3T3 (B); mouse heart (C); rat brain (D) whole cell lysates.

Specifications

Host Rabbit
Method of Purification TNF alpha antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance TNF alpha is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • 

Western blot analysis using TNF alpha antibody (70R-50493)



 | 

Western blot analysis of TNF alpha expression in HeLa (A); NIH3T3 (B); mouse heart (C); rat brain (D) whole cell lysates.
  • 

Immunohistochemical analysis using TNF alpha antibody (70R-50493)



 | 

Immunohistochemical analysis of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
OR
Shipping
View Our Distributors