TNF alpha antibody (FITC) (61R-1176)
Armenian Hamster monoclonal TNF alpha antibody (FITC)
Overview
Overview
| Synonyms | Monoclonal TNF alpha antibody, Anti-TNF alpha antibody, TNF alpha antibody, Tumor Necrosis Factor alpha antibody, Tumor Necrosis Factor alpha antibody, TNF alpha TNF-a antibody, TNF alpha TNF-a antibody, Anti TNF alpha antibody |
|---|---|
| Specificity | Mouse, Rat |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using TNF alpha antibody (FITC) (61R-1176)
Mouse splenocytes were stimulated with ConA for 2 days, followed by Mouse IL-2 protein and Mouse IL-4 protein for 3 days, and re stimulated with immobilized CD3 antibody and soluble CD28 antibody in the presence of Brefeldin A for 5 hours. The cells were surface stained with CD4 antibody (PE) and intracellularly stained with TNF alpha antibody (FITC).
Specifications
| Host | Hamster |
|---|---|
| Clone | TN3-19 |
| Isotype | IgG |
| Tag/Conjugate | FITC |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: =0.5 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor- alpha, but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed. |
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