TNF alpha antibody (PE) (61R-1415)

Mouse monoclonal TNF alpha antibody (PE)

Synonyms Monoclonal TNF alpha antibody, Anti-TNF alpha antibody, Tumor Necrosis Factor alpha antibody, Tumor Necrosis Factor alpha antibody, Anti TNF alpha antibody, TNFa TNF-a antibody, TNF alpha antibody, TNFa TNF-a antibody, TNF-alpha antibody, TNF-alpha antibody
Specificity Human
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest

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Flow Cytometric analysis using TNF alpha antibody (PE) (61R-1415)

Normal human peripheral blood cells were stimulated with PMA and Ionomycin in the presence of Brefeldin A for 5 hours. The cells were surface stained with CD4 antibody (FITC) and intracellularly stained with TNF alpha antibody (PE).

Specifications

Host Mouse
Clone MAb11
Isotype IgG1 kappa
Tag/Conjugate PE
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 200 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.25 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The MAb11 antibody reacts with human tumor necrosis factor- alpha, a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4 T cells. TNF- alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF- alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

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Images

  • Flow Cytometric analysis using TNF alpha antibody (PE) (61R-1415) | Normal human peripheral blood cells were stimulated with PMA and Ionomycin in the presence of Brefeldin A for 5 hours. The cells were surface stained with CD4 antibody (FITC) and intracellularly stained with TNF alpha antibody (PE).

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Price From: Price: €208.59
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