TNF alpha protein (30R-AT013)
Purified recombinantRat TNF alpha protein
Overview
Overview
| Synonyms | TNF-a protein, DIF protein, TNFa protein, Cachectin protein, Differentiation-inducing factor protein, Cytotoxin protein, Tumor Necrosis Factor protein, Necrosin protein, TNFSF2 protein, TNF-alpha protein |
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| Species | Rat |
| Protein Type | Recombinant |
| Applications | User optimized |
Specifications
| Residues | Region of TNF alpha protein corresponding to amino acids MLRSSSQNSS DKPVVHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL. |
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| Expression System | E.coli |
| Grade & Purity | > 98% pure |
| Molecular Weight | 17.3 kDa |
| Form & Buffer | Lyophilized from PBS, pH 7.1. Reconstitute in water to a concentration of 0.1 - 1.0 mg/ml. |
Usage & Assay Information
| Bioactivity | The ED50 as determined by the cytolysis of Mouse L929 cells in the presence of actinomycin D is 5 x 10^7 units/mg. |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Endotoxin Levels | Endotoxin level is less than 0.1 ng per ug (1 EU/ug). |
General Information
| Biological Significance | TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. |
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