TNF beta protein (30R-AT061)
Purified recombinant Human TNF beta protein
Overview
Overview
| Synonyms | TNFb protein, TNF-b protein, Tumor Necrosis Factor-beta protein, Lymphotoxin-alpha protein, TNFSF1 protein, LT-alpha protein, TNF-beta protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | User optimized |
Specifications
| Residues | Region of TNF beta protein corresponding to amino acids GVGLTPSAAQ TARQHPKMHL AHSTLKPAAH LIGDPSKQNS LLWRANTDRA FLQDGFSLSN NSLLVPTSGI YFVYSQVVFS GKAYSPKATS SPLYLAHEVQ LFSSQYPFHV PLLSSQKMVY PGLQEPWLHS MYHGAAFQLT QGDQLSTHTD GIPHLVLSPS TVFFGAFAL. |
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| Expression System | Hi-5 cells |
| Grade & Purity | > 98% pure |
| Molecular Weight | 18.6 kDa |
| Form & Buffer | Lyophilized from 10mM Na3PO4, pH 7.0, with 50 mM NaCl. Reconstitute in Water to a concentration of 0.1-1.0 mg/ml. |
Usage & Assay Information
| Bioactivity | The ED50 was determined by the cytolysis of Mouse L929 cells in the presence of actinomycin D is approximately 0.05 ng/ml, corresponding to a specific activity of approximately 2 x 10^7 units/mg. |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Endotoxin Levels | Endotoxin level is less than 0.1 ng per ug (1 EU/ug). |
General Information
| Biological Significance | TNF-beta is a potent mediator of inflammatory and immune responses. It belongs to the TNF superfamily of ligands and signals through TNFR1 and TNFR2. TNF-beta is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha. Like TNF-alpha, TNF-beta is involved in the regulation of various biological processes incuding cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-beta is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-beta, which effectively anchors the TNF-beta to the cell surface. TNF-beta is cytotoxic to a wide range of tumor cells. |
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