TNF protein (80R-4297)
Purified Recombinant TNF protein (His tagged)
Overview
Overview
| Synonyms | DIF protein, TNF-alpha protein, TNFA protein, TNFSF2 protein |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
SDS-PAGE analysis using TNF protein (His tag) (80R-4297)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 77-233 aa: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIALHHH HHH |
|---|---|
| Expression System | Insect cells |
| Grade & Purity | > 90% pure |
| Molecular Weight | 18.1 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in PBS buffer (pH 7.4) containing 10% glycerol |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage. For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeat freeze/thaw cycles |
|---|---|
| Endotoxin Levels | < 1.0 EU per 1 microgram of protein |
General Information
| Biological Significance | TNF, also known as tumor necrosis factor, is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, under certain conditions it can stimulate cell proliferation and induce cell differentiation. Recombinant human TNF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
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