TNF protein (80R-4297)

Purified Recombinant TNF protein (His tagged)

Synonyms DIF protein, TNF-alpha protein, TNFA protein, TNFSF2 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis using TNF protein (His tag) (80R-4297)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 77-233 aa: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIALHHH HHH
Expression System Insect cells
Grade & Purity > 90% pure
Molecular Weight 18.1 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in PBS buffer (pH 7.4) containing 10% glycerol
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeat freeze/thaw cycles
Endotoxin Levels < 1.0 EU per 1 microgram of protein

General Information

Biological Significance TNF, also known as tumor necrosis factor, is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, under certain conditions it can stimulate cell proliferation and induce cell differentiation. Recombinant human TNF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

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Images

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SDS-PAGE analysis using TNF protein (His tag) (80R-4297)



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Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €275.66
Size: 50 ug
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