TNFalpha protein (30R-1060)

Purified recombinant Human TNFalpha protein

Synonyms TNF protein, TNFA protein, TNF-alpha protein, TNFSF2 protein, Tumor necrosis factor alpha protein, , CACHECTIN protein, DIF (differentiation inducing factor ) protein, DIF protein, Tumor necrosis factor alpha protein, member 2) protein, TNF superfamily member 2 protein, tumor necrosis factor (TNF superfamily protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information L929 cytotoxicity assay using actinomycin D Cell Number: 2 x 10,000 cells/well Incubation: 24hr after sample treatment Assay Media: 5% FBS media Cytokine Concentration: 12 pg/ml - 25 ng/ml Detection method: MTT assay

Images

Coomassie Blue stained SDS-PAGE of TNFalpha protein (30R-1060)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 17.5 kDa (158 aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in PBS, pH 7.4.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance Tumor necrosis factor alpha (TNF-alpha), also called cachectin, consists of 157 amino acids. TNF-alpha is a 17.5 kDa factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-alpha is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Recombinant human TNF-alpha was expressed in E. coli and purified by using conventional chromatography techniques. Additional amino acid, methionine, was attached at N-terminus of the protein.

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Images

  • Coomassie Blue stained SDS-PAGE of TNFalpha  protein (30R-1060) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €235.21
Size: 100 ug
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