TNFbeta/LTA protein (His tag) (80R-1284)

Purified recombinant Human TNFbeta/LTA protein

Synonyms TNF B protein, TNFB protein, member 1) protein, Lymphotoxin alpha (TNF superfamily protein, CLTalpha protein, TNF superfamily member 1 protein, Lymphotoxin alpha, TNFSF 1 protein, DIF protein, Hemorrhagic factor protein, Coley?s toxin protein, LT protein, Natural killer cytotoxic factor protein, Tumor necrosis factor beta protein, OTTHUMP00000037612 protein, LTA protein, NKCF protein, Lymphotoxin alpha protein, LT alpha protein, LTalpha protein, Tumor necrosis factor ligand superfamily member 1 protein, Necrosin protein, Tumor necrosis factor superfamily member 1 protein, Differentiation inducing factor protein, OTTHUMP00000037613 protein, TNFbeta protein, TNFSF1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information L929 cytotoxicity assay using actinomycin D Cell Number: 2 x 10,000 cells/well Incubation: 24hr after sample treatment Assay Media: 5% FBS media Cytokine Concentration: 12 pg/ml - 25 ng/ml Detection method: MTT assay

Images

Coomassie Blue stained SDS-PAGE of TNFbeta/LTA protein (His tag) (80R-1284)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 35-205 amino acids: MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 20.9 kDa (192aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 30% glycerol, 0.1M NaCl, and 1mM DTT.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Recombinant TNF beta protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of TNFbeta/LTA protein (His tag) (80R-1284) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €319.89
Size: 50 ug
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