TNNC1 protein (His tag) (80R-2013)

Recombinant human TNNC1 protein (His tag)

Synonyms TNNC 1, TNNC-1, TNC protein, TNNC-1 protein, TN-C protein, CMH13 protein, TNNC protein, Troponin C type 1 protein, TNNC 1 protein, TNNC1, CMD1Z protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of TNNC1 protein (His tag) (80R-2013)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-161 amino acids: MGSSHHHHHH SSGLVPRGSH MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEFLEFMKGV E
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 20.5 kDa (181aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations TNNC1 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance Troponin C type 1, also known as TNNC1, is a member of troponins family. Troponin has 3 subunits, Troponin I(Tn-I), Troponin T(Tn-T) and Troponin C(Tn-C). Tn-I subunit inhibits actomyosin ATPase and Tn-T subunit binds tropomyosin and Tn-C, while Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. TNNC1 is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Recombinant human TNNC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of TNNC1 protein (His tag) (80R-2013) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €301.63
Size: 50 ug
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