Toll-like receptor 4 antibody (10R-6683)
Rat monoclonal Toll-like receptor 4 antibody
Overview
Overview
| Synonyms | Monoclonal Toll-like receptor 4 antibody, Anti-Toll-like receptor 4 antibody, Toll-like receptor-4, TLR4/ MD-2 antibody, Toll-like receptor 4, Toll-like receptor-4 antibody, Toll-like receptor 4 antibody, toll-like receptor MD2 complex antibody, TLR-4/ MD2 antibody, Toll-like receptor 4 |
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| Specificity | Mouse |
| Applications | User optimized |
| Assay Information | This reagent has been tested in bioassays using the human OB-R transfected mouse BaF/3 cell line. The ED50 measured in the human OB-R transfected mouse BaF/3 cell proliferation assay is typically 1.36 ng/ml, corresponding to a specific activity of approximately 7 x105 Units/mg. |
Images
Flow Cytometric analysis using Toll-like receptor 4 antibody (10R-6683)
Staining of thioglycolate-induced peritoneal exudate cells with TLR4/MD-2 Complex antibody (PE). Appropriate Isotype Controls were used (open histogram). Cells in the large scatter population were used for analysis.
Specifications
| Host | Rat |
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| Clone | MTS510 |
| Isotype | IgG2a kappa |
| Method of Purification | Toll-like receptor 4 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 /MD-2 complex. At least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. In the mouse, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages. Incubation of peritoneal macrophages with LPS results in down regulation of surface TLR4/MD-2. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, both of which have been well characterized as hyporesponders to LPS. The MTS510 monoclonal antibody co-immunoprecipitates MD-2 and TLR4, and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex |
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