Toll-like receptor 9 antibody (10R-6668)

Rat monoclonal Toll-like receptor 9 antibody

Synonyms Monoclonal Toll-like receptor 9 antibody, Anti-Toll-like receptor 9 antibody, Toll-like receptor 9, Toll-like receptor-9 antibody, toll-like receptor 9 antibody, Toll-like receptor-9, Toll-like receptor 9, Toll-like receptor 9 antibody, TLR-9 antibody
Specificity Human
Applications Functional Assay
Assay Information This recombinant mouse VEGF120 has been tested in bioassay for induction of proliferation of human umbilical vein endothelial cells. The ED50 for this effect is typically 8.0 ng/ml.

Images

Flow Cytometric analysis using Toll-like receptor 9 antibody (10R-6668)

HEK 293 parent cells (left panel) and 293/hTLR9 transfected cells (right panel) were fixed Permeabilized and subsequently stained with either Anti-Rat IgG (FITC) alone (thin line) or with 1 ug of CD289 antibody followed by Anti-Rat IgG (FITC) (thick line).

Specifications

Host Rat
Clone 72-1665
Isotype IgG2a kappa
Method of Purification Toll-like receptor 9 antibody was purified by affinity chromatography.
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance 72-1665 is generated against a portion of human toll-like receptor 9, a molecule reported to be expressed predominantly intracellularly. TLR9 is a ~115-120 kDa molecule, which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9-positive human B cells. When stimulated with CpG DNA, TLR9-deficient mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least twelve members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on lysed whole human blood and/or isolated human PBMC stained for cell surface or intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Human pDCs matured in the presence of IL-3 have been reported to stain with 72-1665 by immunofluorescence microscopy. Human Epithelial Cell lines were also reported to stain with this mAb. Further studies are needed to determine the relationship between mRNA expression and protein detection by flow cytometry.

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Images

  • Flow Cytometric analysis using Toll-like receptor 9 antibody (10R-6668) | HEK 293 parent cells (left panel) and 293/hTLR9 transfected cells (right panel) were fixed Permeabilized and subsequently stained with either Anti-Rat IgG (FITC) alone (thin line) or with 1 ug of CD289 antibody followed by Anti-Rat IgG (FITC) (thick line).

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Price From: Price: €149.69
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