Toll-like receptor 9 antibody (10R-6668)
Rat monoclonal Toll-like receptor 9 antibody
Overview
Overview
| Synonyms | Monoclonal Toll-like receptor 9 antibody, Anti-Toll-like receptor 9 antibody, Toll-like receptor 9, Toll-like receptor-9 antibody, toll-like receptor 9 antibody, Toll-like receptor-9, Toll-like receptor 9, Toll-like receptor 9 antibody, TLR-9 antibody |
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| Specificity | Human |
| Applications | Functional Assay |
| Assay Information | This recombinant mouse VEGF120 has been tested in bioassay for induction of proliferation of human umbilical vein endothelial cells. The ED50 for this effect is typically 8.0 ng/ml. |
Images
Flow Cytometric analysis using Toll-like receptor 9 antibody (10R-6668)
HEK 293 parent cells (left panel) and 293/hTLR9 transfected cells (right panel) were fixed Permeabilized and subsequently stained with either Anti-Rat IgG (FITC) alone (thin line) or with 1 ug of CD289 antibody followed by Anti-Rat IgG (FITC) (thick line).
Specifications
| Host | Rat |
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| Clone | 72-1665 |
| Isotype | IgG2a kappa |
| Method of Purification | Toll-like receptor 9 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | 72-1665 is generated against a portion of human toll-like receptor 9, a molecule reported to be expressed predominantly intracellularly. TLR9 is a ~115-120 kDa molecule, which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9-positive human B cells. When stimulated with CpG DNA, TLR9-deficient mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least twelve members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on lysed whole human blood and/or isolated human PBMC stained for cell surface or intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Human pDCs matured in the presence of IL-3 have been reported to stain with 72-1665 by immunofluorescence microscopy. Human Epithelial Cell lines were also reported to stain with this mAb. Further studies are needed to determine the relationship between mRNA expression and protein detection by flow cytometry. |
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