Transferrin antibody (20C-CR6072RP)
Rabbit polyclonal Transferrin antibody
Overview
Overview
| Synonyms | Polyclonal Transferrin antibody, Anti-Transferrin antibody, DKFZp781D0156 antibody, Serotransferrin precursor antibody, Siderophilin antibody, PRO2086 antibody, Apotransferrin antibody, PRO1557 antibody, TF antibody, beta 1 metal binding globulin antibody, PRO1400 antibody |
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| Specificity | human transferrin |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, WB |
| Immunogen | Transferrin antibody was raised in rabbit using human transferrin as the immunogen. |
Images
Western Blot analysis using Transferrin antibody (20C-CR6072RP)
Primary and Dylight conj ugated secondary antibodies were used to detect: Human transferrin (green); alpha 1 Antitrypsin (red); and Human IgG (blue) in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000 in IRdye blocking buffer and incubated
Specifications
| Host | Rabbit |
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| Method of Purification | Transferrin antibody was purified by delipidation and defibrination. |
| Form & Buffer | IgG fraction supplied as a liquid in PBS, pH 7.4 with 0.01% NaN3 |
Usage & Assay Information
| Usage Recommendations | Optimal conditions to be determined by end user |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Transferrins are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. In humans, it is encoded by the TF gene. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). Transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. |
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