TRIM23 antibody (70R-12484)

Affinity purified Rabbit polyclonal TRIM23 antibody

Synonyms Polyclonal TRIM23 antibody, Anti-TRIM23 antibody, E3 ubiquitin-protein ligase TRIM23 antibody, TRIM-23, ARFD1 antibody, TRIM 23 antibody, TRIM23 antibody, tripartite motif containing 23 antibody, tripartite motif-containing 23 antibody, RING finger protein 46 antibody, ARD1 antibody, GTP-binding protein ARD-1 antibody, ARF domain protein 1 antibody, TRIM-23 antibody, ADP-ribosylation factor domain protein 1 64kDa antibody, tripartite motif protein TRIM23 antibody, TRIM 23, RNF46 antibody, TRIM23, tripartite motif-containing protein 23 antibody, ADP-ribosylation factor domain-containing protein 1 antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, IHC-P, WB
Immunogen TRIM23 antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 1 and 44 of TRIM23

Images

Immunofluorescent staining using TRIM23 antibody (70R-12484)

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM23 antibody (Green) at 1:500 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification TRIM23 antibody was purified by antigen-affinity chromatography
Molecular Weight 64 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using TRIM23 antibody (70R-12484)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM23 antibody (Green) at 1:500 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using TRIM23 antibody (70R-12484)</font></div>     | Immunohistochemical staining of paraffin-embedded gastric CA using TRIM23 antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using TRIM23 antibody (70R-12484)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:HeLa S3) using a 10 % SDS PAGE gel and TRIM23 antibody at a dilution of 1:1000

Availability: In stock

Price: €395.09
Size: 100 ul
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