Immunofluorescent staining using TRIM28 antibody (10R-1202)
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-TRIM28 antibody.
TRIM28 antibody
Immunofluorescent staining using TRIM28 antibody (10R-1202)
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-TRIM28 antibody.
Western Blot analysis of immunized recombinant protein using TRIM28 antibody (10R-1202)
Left: TRIM28 staining. Right: Coomassie Blue staining of immunized recombinant protein.
Specifications
Host
Mouse
Clone
TRI5I093
Isotype
IgG1
Method of Purification
TRIM28 antibody was purified using protein G column chromatography from culture supernatant of hybridoma
cultured in a medium containing bovine IgG-depleted
(approximately 95%) fetal bovine serum.
Form & Buffer
TRIM28 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information
This antibody solution contains sodium azide (NaN )
as a preservative. There is a potential hazard that
NaN reacts with copper or lead to produce an explosive
compound. For safe disposal, the vial has to
be washed thoroughly with water. It is strongly adv
General Information
Biological Significance
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.