TRIM28 antibody (10R-1202)

Mouse monoclonal TRIM28 antibody

Synonyms Monoclonal TRIM28 antibody, Anti-TRIM28 antibody, KAP1 antibody, transcriptional intermediary factor 1 beta antibody, TRIM-28 antibody, TRIM28, TRIM 28, RNF96 antibody, TF1B antibody, TRIM-28, TRIM 28 antibody, TIF1B antibody
Specificity Human TRIM28
Cross Reactivity Human, Mouse. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen TRIM28 antibody was raised in mouse using recombinant Tripartite Motif-Containing 28 (Trim28)

Images

Immunofluorescent staining using TRIM28 antibody (10R-1202)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-TRIM28 antibody.

Specifications

Host Mouse
Clone TRI5I093
Isotype IgG1
Method of Purification TRIM28 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer TRIM28 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

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Images

  • Immunofluorescent staining using TRIM28 antibody (10R-1202) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-TRIM28 antibody.
  • Western Blot analysis of immunized recombinant protein using TRIM28 antibody (10R-1202) | Left: TRIM28 staining. Right: Coomassie Blue staining of immunized recombinant protein.

Availability: In stock

Price: €364.83
Size: 100 ug
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