TRXB protein (30R-1540)

Purified recombinant E.coli TRXB protein

Synonyms protein, Thioredoxin reductase protein, FAD/NAD(P)-binding protein, Thioredoxin reductase JW0871 protein, TRXR., ECK0879 protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 0.7ml reaction mixture into a suitable container: The final concentrations are 100mM potassium phosphate, 10mM EDTA, 0.2mM beta-NADPH, 0.05% BSA, 0.014% (w/v) thioredoxin, 5mM DTNB, 5ug, E. coli TRXB. 2. Equilibrate to 25 DEG C and monitor the A412nm until the value is constant using a spectrophotometer. 3. Add 35ul of 100mM DTNB into reaction mixture and mix immediately. 4. Record the increase in A412nm for 2 minutes.

Images

Coomassie Blue stained SDS-PAGE of TRXB protein (30R-1540)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-321 amino acids: MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 34.6 kDa (321aa), confirmed by MALDI-TOF
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is 4-5 units/ml, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance TRXB(Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidative stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E. coli TRXB protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of TRXB protein (30R-1540) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €206.99
Size: 100 ug
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