TSLP protein (30R-2279)
Purified recombinant Human TSLP protein
Overview
Overview
| Synonyms | Thymic Stromal-Derived Lymphopoietin protein |
|---|---|
| Specificity | Human |
| Species | Human |
| Protein Type | Recombinant |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. |
Specifications
| Expression System | Insect cells |
|---|---|
| Grade & Purity | > 95% pure |
| Molecular Weight | 15 kDa |
| Form & Buffer | Supplied as a sterile liquid with 20 mM NaH2PO4, 0.6M NaCl, pH7.2. 0.22 um filtered. |
| Concentration | 5 ul (0.06 ug)/test |
Usage & Assay Information
| Usage Recommendations | FC: 5 ul (0.06 ug)/test |
|---|---|
| Bioactivity | Measured by TARC induction assay by humanPBMCs with an observed ED50 of 10 ng/ml, which corresponds to a specific activity of approximately 1x10^5 Units/mg. |
Storage & Safety
| Storage | Store at -70 deg C or less. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Thymic Stromal Lymphopoietin is a member of the B cell-stimulating factor family. This hemopoietic cytokine was identified from the conditioned medium of a mouse thymic stromal cell line that promoted B-cell development. TSLP is expressed in thymus, spleen, kidney, lung and bone marrow. The bioactivity of TSLP overlaps with that of IL-7, playing a key role in B cell development, as well as stimulating the growth of thymocytes and T cells. Whereas IL-7 facilitates the development of B220+/IgM- pre-B cells, TSLP promotes the development B220+/IgM+ B cells in the mouse. In addition, TSLP stimulates monocytes to produce the T cell-attracting chemokines TARC and MDC. TSLP also activates dendritic cell-primed CD4 T cells to produce Th2 cytokines. TSLP binds to a heterodimeric receptor complex consisting of the IL-7R alpha chain and the TSLP-specific chain to induce STAT3 and STAT5 phosphorylation. Mouse TSLP shares approximately 43% amino acid sequence identity with human TSLP. Recombinant human TSLP is a 15.0 kDa protein consisting of 121 amino acid residues. |
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